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Cloning and Sequence Analysis of Beta 2-Adrenergic Receptor Gene

Liujiao Bian

Open publisher page 2 citations

Abstract

Objective: To clone the full-length gene of beta 2-adrenergic receptor (β2-AR).Method: Primers for PCR were designed according to the porcine β2-AR cDNA sequence in the GenBank.The β2-AR gene was amplified from the total RNA of porcine liver by RT-PCR technology,which was then cloned into pUC18 vector.The recombinant was transformed to competent cell E.coil DH5α,screening the positive clones.Result: The amplified β2-AR gene has 1257 bases,encoding 418 amino acids.Compared with the porcine β2-AR DNA sequence inscribed in GenBank, the homology is 99.52% with 99.04% amino acids identical.Conclusion: Full-length sequence of β2-AR Gene was successfully obtained,establishing foundation for gene expression and the construction of receptor-drugs screening model.

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What this paper is about

Objective: To clone the full-length gene of beta 2-adrenergic receptor (β2-AR).Method: Primers for PCR were designed according to the porcine β2-AR cDNA sequence in the GenBank.The β2-AR gene was amplified from the total RNA of porcine liver by RT-PCR technology,which was then cloned into pUC18 vector.The recombinant was transformed to competent cell E.coil DH5α,screening the positive clones.Result: The amplified β2-AR gene has 1257 bases,encoding 418 amino acids.Compared with the porcine β2-AR DNA sequence inscribed in GenBank, the homology is 99.52% with 99.04% amino acids identical.Conclusion: Full-length sequence of β2-AR Gene was successfully obtained,establishing foundation for gene expression and the construction of receptor-drugs screening model.

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Available abstract

Objective: To clone the full-length gene of beta 2-adrenergic receptor (β2-AR).Method: Primers for PCR were designed according to the porcine β2-AR cDNA sequence in the GenBank.The β2-AR gene was amplified from the total RNA of porcine liver by RT-PCR technology,which was then cloned into pUC18 vector.The recombinant was transformed to competent cell E.coil DH5α,screening the positive clones.Result: The amplified β2-AR gene has 1257 bases,encoding 418 amino acids.Compared with the porcine β2-AR DNA sequence inscribed in GenBank, the homology is 99.52% with 99.04% amino acids identical.Conclusion: Full-length sequence of β2-AR Gene was successfully obtained,establishing foundation for gene expression and the construction of receptor-drugs screening model.

Key concepts: GenBank, Molecular biology, Gene, Sequence analysis, Cloning (programming), Complementary DNA, Biology, Homology (biology)

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