2010•Unpublished venueRequires access
Cloning and expression of porcine interleukin-2 (IL-2) gene.
Chunhua Li, Xizhong He, Zhang Chun-ling, Yong Zou, Fengying Jiang, Jianping Ni
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Abstract
Based on the published nucleotide sequence of porcine interleukin-2(IL-2)gene,a pair of RT-PCR primers were designed and synthesized.The ConA-stimulated porcine peripheral blood lymphocytes were used as materials,and the specific fragments of 484 bp were amplified from the total RNA and cloned into pGEM-T easy vector by RT-PCR.The sequence determination showed that the homology between the gene of the fragment and the porcine IL-2 gene published in GenBank reached 99.8% in nucleotide sequence.The directional cloning of the fragment into the prokaryotic expression vector pET32a constructed a genetically engineered strain expressing recombinant porcine IL-2 gene,and the purified recombinant porcine IL-2 protein was obtained by induction with IPTG and chromatography.