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Cloning and sequencing of porcine interleukin-18 mature protein gene

Wen Na-xiang, Ruiai Chen, Pei Zhang-fu, Tang Man-hua, Xiaoqin Jin, Wei Zhiqing, Zhu Wen-guan

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Abstract

One pair of specific primers to porcine IL-18 protein gene was designed and synthesized (according) to the published sequence in GenBank, and was used to amplify porcine IL-18 cDNA by RT-PCR from total RNA of porcine spleen cells. RT-PCR product was cloned into pMD 18-T vector. The nucleotide acid sequence was determined after being identified by restriction endonucleases digestion. The result show that the full length of porcine IL-18 cDNA is 474 bp, which encodes 157 amino acids.

About this research paper

What this paper is about

One pair of specific primers to porcine IL-18 protein gene was designed and synthesized (according) to the published sequence in GenBank, and was used to amplify porcine IL-18 cDNA by RT-PCR from total RNA of porcine spleen cells. RT-PCR product was cloned into pMD 18-T vector. The nucleotide acid sequence was determined after being identified by restriction endonucleases digestion. The result show that the full length of porcine IL-18 cDNA is 474 bp, which encodes 157 amino acids.

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Available abstract

One pair of specific primers to porcine IL-18 protein gene was designed and synthesized (according) to the published sequence in GenBank, and was used to amplify porcine IL-18 cDNA by RT-PCR from total RNA of porcine spleen cells. RT-PCR product was cloned into pMD 18-T vector. The nucleotide acid sequence was determined after being identified by restriction endonucleases digestion. The result show that the full length of porcine IL-18 cDNA is 474 bp, which encodes 157 amino acids.

Key concepts: Biology, GenBank, Complementary DNA, Cloning (programming), Molecular biology, Restriction enzyme, Gene, Nucleic acid sequence

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