Study on antiproliferative effects of genistein on human endometrial cancer cell line HEC-1B in vivo and in vitro

Objective The inhibitive effects of genistein on the proliferation of endometrial cancer cell line HEC-1B in vitro and in vivo were investigated,and its anticancer mechanism were explored.Methods MTT,HE stain of histopathologic slides and others were used to assay the inhibitive effects of genistein on the proliferation of endometrial cancer cell line HEC-1B in vitro and in vivo,respectively.Results In vitro,genistein ranged from 0 to 25μmol/L in the medium had a marked promotion on the proliferation of HEC-1B cells(P 0.05).However,it exerted an inhibitive action on the cancer cells at the concentration of 50μmol/L,and a significant antiproliferative effect occurred at 100μmol/L with a fifty-five percent of inhibition rate(P 0.05).In vivo,after the treatment of 100μmol/L genistein,the average tumor weight of nude mice injected by HEC-1B cells remarkably reduced(P 0.05),with its inhibition rate by thirty-nine percent,the decreased number of microvessel and obvious necrosis was found in tumor tissues under a microscope.Conclusions Genistein could effectively inhibit the proliferation of endometrial cancer cell line HEC-1B and its induced tumors in nude mice,and the anticancer mechanism was presumed to antagonize estrogen to bind estrogen receptors,them the proliferative and tumor angiofenesis effects of estrogen were inhibited,which result in the necrosis of the cells finally.