Influence of genistein on extracorporeal proliferation of endometrial cancer cell

Objective To study the inhibitory mechanism of genistein on extracorporeal proliferation of ishikawa cells of human endometrial cancer cell line. Methods Ishikawa cells of human endometrial cancer cell line were cultured in vitro and then the proliferation of cells by MTT method and cell cycle was detected by flow cytometry. Result Genistein inhibited the proliferation of ishikawa cells in vitro with a characteristic of dose-dependence. The higher of genistein concentration was, the more remarkable of genistein effect was. The inhibitory effect was most obviously 48 hours after the administration of genistein. After 72 hours the inhibitory rate was increased slightly without significance and after 96 hours it was decreased slightly. The inhibitory rate was significantly decreased 120 hours after the administration of genistein. Ishikawa cells effected by genistein were blocked in G2-M phase significantly. Conclusion Genistein can inhibit the extracorporeal proliferation of ishikawa cells through blocking the cells in G2-M phase.