Simultaneous determination of 3 components in Lianju Ganmao capsule by UPLC

Objective: To establish an ultra performance liquid chromatography(UPLC) method for simultaneous determination of 3 components in Lianju Ganmo capsule.Methods: The analyses were performed on Waters Acquity UPLC system.An Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm) was used for all analyses.The investigated compounds were separated with gradient mobile phase consisting of 0.1% phosphoric acid and acetonitrile.The flow velocity was 0.2 mL·min-1.The temperature of sample manager was set at 45℃.UPLC-PDA system was used to analysis the sample which the sample mix in 50% ethanol through ultrasound.The detection wavelength was 325 nm.Results: The investigated compounds including 1,5-O-dicaffeoyl-quinic acid 3,5-O-dicaffeoyl-quinic acid 3,4-O-dicaffeoyl-quinic acid,had a good linearity(r≥0.999 8) over the tested ranges.The average recovery was 99.1% to 101.7% with RSD≤2.5%.Conclusion: The developed UPLC method is simple,sensitive and accurate with good repeatability,which is available for quality control of Lianju Ganmao capsule.