ScholarLens Back to search
2016•Journal of Guangdong Pharmaceutical UniversityRequires access

Simultaneous determination of four components in compound Salvia tablets by UPLC analysis

WU Liron

Open publisher page 0 citations

Abstract

Objective To develop a novel and rapid method with ultra-performance liquid chromatography for the simultaneous determination of four saponins in compound Salvia tablets. Methods An acquity UPLC BEH C18( 2.1 mm×50 mm,1.7 μm) was used. Acetonitrile and water were adopted with gradient elution of0. 5 m L / min,and the detection wavelength was 203 nm. The column temperature was 30 ℃. Results Notoginsenoside R1 and ginsenoside Rg1,Re and Rb1 in compound Salvia tablets had good linearity. And the average recoveries were among 98. 9%- 102. 2%( n = 6). Conclusion The relative deviation of the content was 0.01% when UPLC and HPLC was used to determine the same sample. UPLC could greatly improve the separation efficiency and reduce the solvent consumption. As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.

About this research paper

What this paper is about

Objective To develop a novel and rapid method with ultra-performance liquid chromatography for the simultaneous determination of four saponins in compound Salvia tablets. Methods An acquity UPLC BEH C18( 2.1 mm×50 mm,1.7 μm) was used. Acetonitrile and water were adopted with gradient elution of0. 5 m L / min,and the detection wavelength was 203 nm. The column temperature was 30 ℃. Results Notoginsenoside R1 and ginsenoside Rg1,Re and Rb1 in compound Salvia tablets had good linearity. And the average recoveries were among 98. 9%- 102. 2%( n = 6). Conclusion The relative deviation of the content was 0.01% when UPLC and HPLC was used to determine the same sample. UPLC could greatly improve the separation efficiency and reduce the solvent consumption. As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To develop a novel and rapid method with ultra-performance liquid chromatography for the simultaneous determination of four saponins in compound Salvia tablets. Methods An acquity UPLC BEH C18( 2.1 mm×50 mm,1.7 μm) was used. Acetonitrile and water were adopted with gradient elution of0. 5 m L / min,and the detection wavelength was 203 nm. The column temperature was 30 ℃. Results Notoginsenoside R1 and ginsenoside Rg1,Re and Rb1 in compound Salvia tablets had good linearity. And the average recoveries were among 98. 9%- 102. 2%( n = 6). Conclusion The relative deviation of the content was 0.01% when UPLC and HPLC was used to determine the same sample. UPLC could greatly improve the separation efficiency and reduce the solvent consumption. As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.

Key concepts: High-performance liquid chromatography, Chromatography, Salvia, Acetonitrile, Chemistry, Gradient elution, Solvent, Relative standard deviation

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Simultaneous determination of four components in compound Salvia tablets by UPLC analysis — Research Paper | ScholarLens