Analysis of relationship between HBV-DNA level and serum markers

Objective To analyze the relationship between the level of HBV-DNA and HBV-M.Methods 437 outpatients and inpatients in our hospital from January to December 2011 were detected HBV-DNA and HBV-M by fluorescence quantitative PCR and ELISA assay and the results were analyzed.Results Among 437 specimens,136 cases were positive HBsAg(+),HBeAg(+) and HBcAb(+),and the positive rate of serum HBV-DNA was 98.5%,the content was 7.07±1.31.175 cases were positive HBsAg(+),HBeAb(+) and HBcAb(+),the positive rate of serum HBV-DNA was 72.0%,the content was 5.27±1.46.67 cases were positive HBsAg(+) and HBcAb(+),the positive rate of serum HBV-DNA was 58.2%,the content was 5.11±1.60.Conclusion A close correlation exists between these two methods in the detection of HBV markers.The FQ-PCR is a relatively accurate method for detecting HBV-DNA with high sensitivity and strong specificity,which can correctly reflect the replication level and activity degree of HBV.HBeAg,PreS1 and other serum markers and quantitative determination of HBV-DNA are the important method of HBV infection.