Investigation of Radio-Immunity Method and FQ-PCR in Checking Hepatitis B Virus

Objective:To investigate the relationship between HBV-DNA determined by the polymerase chain reaction(PCR)assay and Hepatitis-B marke-rs by RIA method.Methods:The serum samples from 323 cases with 8 different positive models were tested by Fluorescence quantitative PCR assay(FQ-PCR)and also by RIA to compare the results.Results:Among the patients,the positive rate of HBV-DNA for the patients with positive HBsAg、HBc-Ab and HBeAe was 100%;the positive rate of HBV-DNA for the patients with positive HBsAg、HBcAb and HBsAb was 44.92%;the positive rate of HBV-DNA for the patients with positive HBsAb、HBcAb、HBeAe and HBeAb was 98.08%;the positive rate of HBV-DNA for the patients with positive HBsAb and HBcAb was 50%;the positive rate of HBV-DNA for the patients with positive HBsAb、HBcAb and HBeAb was 0%;the positive rate of HBV-DNA for the patients with positive HBcAb and HBeAb was 12.12%;the positive rate of HBV-DNA for the patients with positive HBcAb was 0%;the positive rate of HBV- DNA for the patients with all negative HBVm was 0%.Conclusione:HBV-DNA determined by PCR assay is much more corrct and sensitive than HBV-markers by RIA ass-ay.FQ-PCR could be used as a excellent monitor for the state of HBV infection and its complication,especially for the state of low complication.