Correlative Analysis of Results between HBV-DNA with Real-time Fluorescent Quantitative PCR Method and HBV Immune Markers with ELISA in Serum

Objective To explore the application in clinical diagnosis with analying of results between HBV-DNA with real-time fluorescent quantitative PCR method and HBV immune markers with ELISA in serum. Methods Real-time PCR detected HBV-DNA, ELISA assay of serum HBV immune markers. Results 121 patients with hepatitis B samples, HBsAg+, HBeAg+, HBcAb+ positive patients, 39 patients, serum HBV-DNA positive rate was 67%, the average concentration of 1.42 × 107 IU/ml; HBsAg+, HBeAb+, HBcAb+ positive patients had 64 cases serum HBV-DNA positive rate of 45%, the average concentration of 4.29 × 105 IU/ml; HBsAg+, HBcAb-positive patients in 6 patients, serum HBV-DNA quantitative values are 500; HBsAg+, HBeAg+, HBeAb+, HBcAb+ 2 cases serum HBV-DNA positive 50%, the average concentration of 2.96 × l04 IU/ml. Conclusion Results of HBV-DNA with real-time PCR and HBV immune markers can provide accurate and reliable data for clinical diagnosis.