The role of survivin in the apoptosis of cervical cancer cell line induced by cisplatin

Objective To study the role of survivin in the apoptosis of cervical cancer cell line induced by cisplatin (DDP). Methods The inhibition effects of different doses of DDP on Hela cell line were assayed with MTT test. Cultured Hela cells were divided into 2 groups: DDP and control. DDP group was divided into 10, 4, 2, 1, 0.1, 0.01 mg/L groups. The expression of survivin was determined by RT-PCR and Western blotting on 24, 48, 72 and 96 h after Hela cells exposed to cisplatin. Apoptosis and cell cycle changes were determined by flow cytometry. Results DDP obviously inhibited the cervical cancer cell growth in a manner of dose- and time-dependent. Apoptosis induced by chemotherapeutical drug increased at first and then decreased as the concentrations of DDP got higher while as the time went by, the apoptosis in the high DDP concentration group increased significantly but in the low DDP concentration group increased slowly. As time went on, survivin mRNA and protein level were increased after Hela cells exposed to 0.1 mg/L and 1 mg/L of DDP. Conclusion A high expression of survivin was induced in Hela cells by low concentration of cisplatin in a time-dependent manner, which may be caused by chemoresistence of cervical carcinoma cells induced by chemotherapy agent.