2002•Harbin Yike Daxue xuebaoRequires access
Ooning, sequencing and construction of eukaryotic expression vector for B7-1 gene
Dai Wenjie
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Abstract
Objective To clone, sequence and construct eukaryotic expression vector of mouse B7-1 gene. Methods The total RNA was isolated from mouse spleen.The B7-1 cDNA was synthesized and amplied from the mRNA by RT-PCR and the PCR product was cloned into pcDNAS eukaryotic expression vector and subjected to DNA sequencing. Results Sequencing analysis revealed that the amino acid sequence of mouse B7-1 had one variation in nucleotide sequence which caused one amino acid residue displace( compared with that of published sequence). Conclusion We successfully construct the eukaryotic expression vector of mouse B7-1 gene, which provide a solid basis for the study of B7-1 on antitumor genetherapy.