Cloning,sequencing and construction of eukaryotic expression vector for mouse angiostatin gene

Objective To clone,sequence and construct eukaryotic expression vector of mouse angiostatin gene.Methods The total RNA was isolated from mouse liver.The angiostatin cDNA was synthesized and amplified from the mRNA by RT PCR and the PCR product was cloned into pcDNA3 eukaryotic expression vector and subjected to DNA sequencing.Results Sequencing analysis revealed that the amino acid sequence of mouse angiostatin have four variations in nucleotide sequence which bring out three amino acid residue displaces(compared with that of published sequence).Conclusion We successfully construct the eukaryotic expression vector of mouse angiostatin gene ,which provide a solid basis for the study of angiostatin on antitumor genetherapy.