Cloning of region gene of IL-37b and its eukaryotic expression vector construction

Objective To clone the coding region gene of IL-37b and to construct the eukaryotic expression vector of IL-37b. Methods The coding region gene of IL-37b was amplified by RT-PCR,and the plasmid of positive clone was isolated after identification by DNA sequencing. The target gene was sub-cloned into the eukaryotic expression vector pcDNA3. 1,which was identified by PCR and DNA sequencing. Results A specific DNA band about 650 bp of RT-PCR product was seen by agarose electrophoresis,and DNA sequencing result showed that IL-37b gene of 654 bp was achieved. After construction of the eukaryotic expression vector was identified by PCR and DNA sequencing,the result showed that coding region gene of IL-37b was inserted into the eukaryotic vector pcDNA3. 1. Conclusion The coding region gene of IL-37b is successfully cloned as well as its eukaryotic expression vector,which lays a foundation for further research of the biology role of IL-37b.