Gene Cloning of Human Interleukin-37b and Construction of Its Eukaryotic Expression Vector

Objective:To clone the gene sequence encoding human interleukin( IL)-37b gene and construct its eukaryotic expression vector.Method:Total RNA was extracted from the human prepuce and the gene fragment encoding IL-37b gene was amplified by reverse transcriptase-PCR using specific primers and then cloned into pcDNA3 vector.The eukaryotic expression plasmid pcDNA3.1-hIL-37b was constructed and transformed into the E.coli for identification.The inserted IL-37b cDNA was sequenced.Result:DNA sequencing showed that the cDNA of IL-37b had the full length of human IL-37b gene consists of 657 bp encoded the product of 218 amino acids.The cDNA shared a high nucleotide homology with that reported previously.Conclusion:We have successfully cloned the full-length cDNA of human IL-37b,which has paved the way for further studying on its biological function.