Callus Induction and Differentiation of Xinjiang Medicago falcata L.

Xinjiang wild Medicago falcata L.was selected to study callus induction,differentiation and propagation regeneration.The influence factors of wild M.falcata L.tissue culture such as hard seed,explants,hormones(2,4-D and KT) and media were discussed.Results showed that the combination of 4℃ low-temperature treatment and sulfuric acid soaking as the better method of breaking the hard seed of M.falcata L..This treatment resulted in 90% germination rate.Hypocotyl tissue was the most suitable explant for callus induction.The MS medium was more effective than the improved SH medium for the callus induction.The optimal callus induction medium contained MS+2,4-D 0.5 mg·L-1+KT 0.8 mg·L-1+sucrose 30 g·L-1+agar powder 7 g·L-1 with 80% callus induction rate.Light green and light yellow callus were differentiated in culture medium containing MS+2,4-D 0.2 mg·L-1+KT 0.4 mg·L-1+CH 1 g·L-1+sucrose 20 g·L-1+agar 7 g·L-1 with 30% differentiation rate.This technique will lay a foundation for M.falcata L.biotechnology breeding.