Tissue Culture and Regeneration System of Native Medicago falcata L.

Using cotyledons and hypocotyls of wild Medicago falcata L.from Hulunbeier grassland as explants,tissue culture and regeneration system of Medicago falcata L.were studied for further transgenic experiments providing a good receptor.Results showed that the optimal medium for callus induction was MS+1.5 mg·L-1 2,4-D+0.4 mg·L-1 6-BA+3% sucrose+0.7% agar,the highest callus induction ratio was 100%.The optimal medium for embryogenic callus was MS+0.5 mg·L-1 KT+0.1 mg·L-1 NAA+2% sucrose+0.7% agar,inducted ratio of embryogenic callus was 81.5%.The optimal medium for differentiated buds was MS+0.25 mg·L-1 KT+0.05 mg·L-1 NAA+2% sucrose+0.7% agar,differentiated ratio of buds was 36.5%.The optimal rooting medium was 1/2MS+0.5 mg·L-1 NAA+1.5% sucrose+0.7% agar,rooting ratio was 93.3%.Callus induction results showed that the tissue culture of Medicago falcata L.had different induction ratio between individuals with the same hormone.