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2011•Unpublished venueRequires access

Determination of salvianolic acid B in Naotong Granula by HPLC

Yang Chun-hui

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Abstract

Objective To establish a method for HPLC determination of salvianolic acid B in Naotong Granula. Methods The condition for HPLC was Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with methanol-acetonitrile-formic acid-water (30∶10∶0.5∶59.5) as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 40 ℃. The injection volume was 10 μL, and detection wavelength was 286 nm. Results The linear range was of 0.441 6—2.208 0 μg for salvianolic acid B and the correlation coefficient was 0.999 7. The average recovery was 98.65% with RSD 1.71%. Conclusion The method is reproducible, reliable, and can be used for quality control of Naotong Granula.

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What this paper is about

Objective To establish a method for HPLC determination of salvianolic acid B in Naotong Granula. Methods The condition for HPLC was Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with methanol-acetonitrile-formic acid-water (30∶10∶0.5∶59.5) as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 40 ℃. The injection volume was 10 μL, and detection wavelength was 286 nm. Results The linear range was of 0.441 6—2.208 0 μg for salvianolic acid B and the correlation coefficient was 0.999 7. The average recovery was 98.65% with RSD 1.71%. Conclusion The method is reproducible, reliable, and can be used for quality control of Naotong Granula.

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Available abstract

Objective To establish a method for HPLC determination of salvianolic acid B in Naotong Granula. Methods The condition for HPLC was Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with methanol-acetonitrile-formic acid-water (30∶10∶0.5∶59.5) as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 40 ℃. The injection volume was 10 μL, and detection wavelength was 286 nm. Results The linear range was of 0.441 6—2.208 0 μg for salvianolic acid B and the correlation coefficient was 0.999 7. The average recovery was 98.65% with RSD 1.71%. Conclusion The method is reproducible, reliable, and can be used for quality control of Naotong Granula.

Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Formic acid, Correlation coefficient, Linear correlation, Linear range, Methanol

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