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2012•China Modern MedicineRequires access

Determination of paeoniflorin in qushihuayu oral liquid by HPLC

Cui Hong-bin

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Abstract

Objective To establish a HPLC method for the determination of paeoniflorin in qushihuayu oral liquid. Methods The chromatographic system consisted of Agilent Eclipse Plus-C18 column, the mobile phase was acetonitrile-0.1% phosphate (14∶86); the column temperature was 30℃; the measurement wavelength was 230 nm and the flow rate was 0.6 mL/min. Results The linear ranges of paeoniflorin was 0.48-9.12 μg(r = 0.999 9), the average recovery was 98.93% and RSD was 0.34% (n = 6). Conclusion The method is simple, rapid, accurate and reliable, good repeatability, it can be used for the determination of paeoniflorin in qushihuayu oral liquid.

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What this paper is about

Objective To establish a HPLC method for the determination of paeoniflorin in qushihuayu oral liquid. Methods The chromatographic system consisted of Agilent Eclipse Plus-C18 column, the mobile phase was acetonitrile-0.1% phosphate (14∶86); the column temperature was 30℃; the measurement wavelength was 230 nm and the flow rate was 0.6 mL/min. Results The linear ranges of paeoniflorin was 0.48-9.12 μg(r = 0.999 9), the average recovery was 98.93% and RSD was 0.34% (n = 6). Conclusion The method is simple, rapid, accurate and reliable, good repeatability, it can be used for the determination of paeoniflorin in qushihuayu oral liquid.

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Available abstract

Objective To establish a HPLC method for the determination of paeoniflorin in qushihuayu oral liquid. Methods The chromatographic system consisted of Agilent Eclipse Plus-C18 column, the mobile phase was acetonitrile-0.1% phosphate (14∶86); the column temperature was 30℃; the measurement wavelength was 230 nm and the flow rate was 0.6 mL/min. Results The linear ranges of paeoniflorin was 0.48-9.12 μg(r = 0.999 9), the average recovery was 98.93% and RSD was 0.34% (n = 6). Conclusion The method is simple, rapid, accurate and reliable, good repeatability, it can be used for the determination of paeoniflorin in qushihuayu oral liquid.

Key concepts: Paeoniflorin, Chromatography, Medicine, High-performance liquid chromatography, Repeatability, Phosphate buffered saline, Chemistry

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