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Content Determination of Paeoniflorin in Kuanxin Oral Solution by HPLC

Liu Xiaohong

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Abstract

OBJECTIVE:To establish the method for the content determination of paeoniflorin in Kuanxin oral solution.METHODS:HPLC method was used.The determination was performed on Eclipse XDB-C18(150 mm×4.6 mm,5 μm)with mobile phase consisted of methanol-water(30:70) at flow rate of 1.0 mL·min-1.The detection wavelength was set at 230 nm and column temperature was 30 ℃.RESULTS:The linear range of paeoniflorin was 0.031 3～1.000 0 μg(r=0.999 9,n=6) with an average recovery rate of 99.63%(RSD=0.67%,n=9).CONCLUSION:The method is simple,accurate and reproducible for the quality control of Kuanxin oral solution.

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OBJECTIVE:To establish the method for the content determination of paeoniflorin in Kuanxin oral solution.METHODS:HPLC method was used.The determination was performed on Eclipse XDB-C18(150 mm×4.6 mm,5 μm)with mobile phase consisted of methanol-water(30:70) at flow rate of 1.0 mL·min-1.The detection wavelength was set at 230 nm and column temperature was 30 ℃.RESULTS:The linear range of paeoniflorin was 0.031 3～1.000 0 μg(r=0.999 9,n=6) with an average recovery rate of 99.63%(RSD=0.67%,n=9).CONCLUSION:The method is simple,accurate and reproducible for the quality control of Kuanxin oral solution.

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Available abstract

OBJECTIVE:To establish the method for the content determination of paeoniflorin in Kuanxin oral solution.METHODS:HPLC method was used.The determination was performed on Eclipse XDB-C18(150 mm×4.6 mm,5 μm)with mobile phase consisted of methanol-water(30:70) at flow rate of 1.0 mL·min-1.The detection wavelength was set at 230 nm and column temperature was 30 ℃.RESULTS:The linear range of paeoniflorin was 0.031 3～1.000 0 μg(r=0.999 9,n=6) with an average recovery rate of 99.63%(RSD=0.67%,n=9).CONCLUSION:The method is simple,accurate and reproducible for the quality control of Kuanxin oral solution.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Linear range, Recovery rate, Linear relationship

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