2009•Zhongguo yaofangRequires access

Determination of Hesperidin in Yangweishu Tablets by RP-HPLC

Wei Xue-jun

Abstract

OBJECTIVE: To establish a RP-HPLC method for the determination of the content of hesperidin in Yangweishu tablets.METHODS: The samples were separated on octadecyl silane bonded gel silica column(150 mm×4.6 mm,5 μm) with the mobile phase consisted of methanol-7% acetic acid solution(32∶68) under a detection wavelength of 283nm.RESULTS: The linear range of hesperidin was 49.6～892.8 ng(r=0.999 9) with an average recovery rate of 99.02%(RSD=0.45%,n=9).CONCLUSION:The method is simple,accurate,reproducible and applicable for the quality control of Yangweishu tablets.

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Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Linear range, Acetic acid, Silica gel