RP-HPLC determination of content of hesperidin in Chenxianglubailu tablets
Fangming Liu
Abstract
Fangming Liu
Abstract
To establish a method for determination of content of hesperidin in Chenxianglubailu tablets. Method:A Diamonsil C_(18)column (4.6 mm×150 mm,5μm) was used with methonal-acetic acid-water (35:4: 61)as mobile phase.The flow rate was 1.0 mL·min~(-1) and detecting wavelength was 284 nm.Results:The linear range was 0.21-2.3μg(r=0.9999)and average recovery was 98.5 %,RSD=0.89% (n=5).Conclusion:The method is simple,fast and accurate.
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To establish a method for determination of content of hesperidin in Chenxianglubailu tablets. Method:A Diamonsil C_(18)column (4.6 mm×150 mm,5μm) was used with methonal-acetic acid-water (35:4: 61)as mobile phase.The flow rate was 1.0 mL·min~(-1) and detecting wavelength was 284 nm.Results:The linear range was 0.21-2.3μg(r=0.9999)and average recovery was 98.5 %,RSD=0.89% (n=5).Conclusion:The method is simple,fast and accurate.
Key concepts: Hesperidin, Chemistry, Chromatography, High-performance liquid chromatography, Linear range, Linear relationship, Acetic acid, Content determination