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2010•Northern HorticultureRequires access

Effects of Different Culture Medium on Callus Induction of L-402 Tomato

Gong Jing-li

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Abstract

In the process of tomato tissue culture,the degree of callus formation ease or difficult and the number of callus directly affected the success of tomato tissue culture.So in the study,choosing leaves of L-402 Tomato as explants,by 4 level concentration IAA and 5 level concentration 6-BA to do comparison test,to find the way of inoculate transgenic callus.The results showed that its effect was the best that using 0.2 mg/L IAA level and 1.5 mg/L 6-BA level inducted callus of L-402 Tomato.

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What this paper is about

In the process of tomato tissue culture,the degree of callus formation ease or difficult and the number of callus directly affected the success of tomato tissue culture.So in the study,choosing leaves of L-402 Tomato as explants,by 4 level concentration IAA and 5 level concentration 6-BA to do comparison test,to find the way of inoculate transgenic callus.The results showed that its effect was the best that using 0.2 mg/L IAA level and 1.5 mg/L 6-BA level inducted callus of L-402 Tomato.

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Available abstract

In the process of tomato tissue culture,the degree of callus formation ease or difficult and the number of callus directly affected the success of tomato tissue culture.So in the study,choosing leaves of L-402 Tomato as explants,by 4 level concentration IAA and 5 level concentration 6-BA to do comparison test,to find the way of inoculate transgenic callus.The results showed that its effect was the best that using 0.2 mg/L IAA level and 1.5 mg/L 6-BA level inducted callus of L-402 Tomato.

Key concepts: Callus, Explant culture, Tissue culture, Botany, Horticulture, Biology, Chemistry, In vitro

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