The expression and clinical significance of VEGF、p53 and MVD in gastric cancer

Objective:To investigate the expression and correlation of VEGF,p53 and MVD in human gastric cancer and discuss their significance in carcinogenesis,progression,invasion and matestasis of gastric cancer,as well as their relation with biological behaviour of gastric cancer.Methods:The expression of VEGF was evaluated in the serum samples of 73 preoperation gastric cancer patients as well as 20 serum samples of healthy persons using ABC-ELISA.The expression of VEGF,p53 and CD34 were studieded in the tissues of gastric cancer and normal tissue near cancer using immunohistochemistry.Results:The expression levels of VEGF in serum of preoperation gastric cancer patients were significantly higher than those in healthy persons(VEGF:101.8±53.3 ng/L vs 16.1±22.5 ng/L,P0.05).The expression levels of VEGF in serum of preoperation gastric cancer patients were positively ralated to invasive depth,TNM stage,metastasis of lymph node and distant metastasis(P0.05).but not with age,sex and pathological type.The expession rate of VEGF in gastric cancer tissues was 71.2%,while was 0 in the tissues near gastric cancer.There was significant difference between two groups(P0.05).The expression of VEGF in gastric cancer tissues was correlated with invasive depth,TNM stage,metastasis of lymph node and distant metastasis(P0.05).but not with age,sex and pathological type.The expression levels of VEGF in serum of preoperation gastric cancer patients were positively correlated with that in gastric cancer tissues(r=0.346,P0.01).The expession of p53 in gastric cancer tissues was significantly higher than that in normal tissues near gastric cancer(P0.05).The expression of p53 in gastric cancer tissues was significantly correlated with the metastasis of lymph node,TNM stage and distant metastasis(P0.05).but not with invasive depth,age,sex and pathological type(P0.05).3 The MVD in gastric cancer tissues was significantly higher than that in normal tissues near gastric cancer.The MVD in gastric cancer tissues was significantly correlated with the invasive depth,metastasis of lymph node,TNM stage and distant metastasis(P0.05).but not correlated with age,sex and pathological type(P0.05).The expression of VEGF in serum of preoperation gastric cancer patients and in gastric cancer tissues was positively correlated with the expession of p53 respectively(r= 0.316,P0.01 ;r=0.290,P0.05).The expression of VEGF in serum of preoperation gastric cancer patients and in gastric cancer tissues was positively correlated with the MVD respectively too(r= 0.434,P0.01 ;r=0.512,P0.01).The expession of p53 was positively correlated with the MVD(r= 0.431,P0.01).Conclusion:VEGF,p53 and MVD might play important roles in the development,progression,metastasis and prognosis of human gastric cancer.They may be new markers for diagnosis and prognosis of gastric cancer.