THE DETERMINATION OF SERUM VASCULAR ENDOTHELIAL GROWTH FACTOR AND MICROVESSEL DENSITY IN GASTRIC CANCER

Objective:To study the relationship between serum vascular endothelial growth factor(VEGF),microvessel density(MVD) and their clinical pathologic feature in patients with gastric cancer.Methods:Enzyme-linked immunosorbent assay(ELISA) and immunohistochemical staining were used to detect serum VEGF concentration and MVD of tumor tissue in 42 cases with gastric cancer.Use mouse ante-human mocolonal antibody to detecte the MVD in castric carcinoma with immunohis to chemistry.Results:Serum VEGF was found to be significantly higher in patients with gastric cancer than that in control group.More significant difference was observed in MVD of tumor tissue than that of normal tissue in patients with gastric cancer(P0.01).There were positive correlation between VEGF and MVD in patients with gastric cancer(r=0.875).VEGF in serum and MVD in tumor tissue of gastric cancer were correlated with lymph node metastasis(P0.05),depth of invasion(P0.01) and histological stage(P0.05),but there was no relations to age and sex(P0.05).Conclusion:VEGF is significantly related to angiogenesis of gastric cancer,Serum VEGF of gastric cancer may be an important index for evaluation of recurrence,metastasis and prognosis for patients.