Optimization of Separation and Purification Technology for Total Flavonoids from Citrus aurantium by AB-8 Macroporous Adsorption Resin

Objective:To optimize separation and purification technology of total flavonoids from Citrus aurantium by AB-8 macroporous resin.Method:Adsorption property of seven different kinds of macroporous resins for total flavonoids from C.aurantium was compared by static adsorption test,in order to select optimum macroporous resin;Single factor test was used to investigate adsorption and elution conditions of macroporous resin.Result:AB-8 macroporous resin was the most suitable one for purification of total flavonoids in C.aurantium;Its optimum separation and purification parameters were as followings:the sample concentration 0.408 g · L-1,pH of 4,sample flow rate 2 BV · h-1,eluted with 6 BV 75% ethanol at 1 BV · h-1;Under this condition,purity of total flavonoids would be increased from 14.03% to 42.54%.Conclusion:Optimized separation and purification technology was stable and feasible,it could be extended to industrial production.