Cloning and sequence analysis of full-length cDNA of EMF2 gene in Camellia oleifera

EMF2 gene maintains vegetative development by repressing flowering program,and plays an important role in the process of flower organ development.In order to offer a reference for the researches on epigenetic regulation of Camellia oleifera characters,the full-length cDNA of EMF2 gene in C.oleifera(Huaxin) was cloned and analyzed by 5′RACE and 3′RACE techniques.The results show that the full length of the gene is 2 210 bp,the ORF box included 1 866 bp and encodes 621 amino acids,and it is named Co-EMF2.The results of multiple sequence alignment show that Co-EMF2 has the typical conserved sequences of EMF2 and relevant characters,and it has high homology with EMF2 in other plant species.the protein is a labile and hydrophobic nuclear protein,and its lsoelectric point is 6.17.