Cloning and sequence analysis of a fatty acid hydroperoxide lyase gene from Camellia oleifera

A cDNA library and a of Camellia oleifera were constructed.According to the fatty acid hydroperoxide lyase EST sequences of Camellia oleifera,a specific primer was designed,the fatty acid hydroperoxide lyase gene was cloned from Camellia oleiferawith 3'RACE and 5'RACE,respectively. This gene is 16 48 bp in length,including 51 bp of 5' untranslated region and 121 bp of 3' untranslated region. It has an open reading frame of 1 476 bp,encoding aprotein of 491 amino acid residues with protein molecular weight of 54 777.9 Da and isoelectric point of 6.77. A chloroplast transfer peptide was predicted,contains 22 hydrophobic amino acid residue in N-terminal,rich in serine and praline,however,the latter is rarely in transfer peptide.The alignment of amino acids sequence reveals that the comparability of Camellia oleifera and Camellia sinensis is 96%. This gene is named coHPL.