THE EFFECT OF ALKYL-LYSOPHOSPHOLIPID (ET-18-OCH_3) ON U266 CELLS

To study the anti tumor effects of alkyl lysophospholipid ET 18 OCH 3 (ALP) on U266 cells. The antiproliferative activity of ET 18 OCH 3 was measureed by the inhibitory rate and colony formation assays of U266 cells during the incubation with ET 18 OCH 3. The effect of ET 18 OCH 3 in apoptosis induction was determined by the observation of morphology, agarose gel electrophoresis, and flow cytometry. The expression of bcl 2?c myc mRNA and proteins were detected by RT PCR and flow cytometry in order to understand the mechanism of apoptosis induction. Results showed that ET 18 OCH 3 owned significant cytotoxity on U266 cells and inhibitory effect on colonies formation. These effects were time and dose dependent. ET 18 OCH 3 could also induce time dependent apoptosis, which was confirmed by morphology characteristics and DNA fragments ladder on electrophoretogram. The apoptosis rate in flow cytometry was 17.53%, with no apoptotic cells found in control. RT PCR showed bcl 2 and c myc mRNA decreased by 86% and 72% respectively after U266 cells were incubated with ET 18 OCH 3 for 24h. In the mean time, the percentage of bcl 2?C myc positive cell decreased by 17% and 60%, respectively. These results suggest that ET 18 OCH 3 has significant inhibitory effect on growth of U266 cells, possibly due to its apoptosis induction in U266 cells.