Apoptosis and bcl-2/bax expression of U251 cell line induced by Fraction C from Naja Naja AtraVenom (FC) in vitro

Objective To study the mechanism of apoptosis induced by Fraction C from Naja Naja Atra Venom (FC) in human glioma cell line U251. Methods U251 cells were treated with Aidi that have been prepared with 1.5 mg/L (group A), 3.0 mg/L (group B), 4.5 mg/L/ml (group C), 15.0 mg/L (group D) and DDP (40 mg/L, group E) as positive control group. U251 cells nottreated with drugs served as negative control group. The change of U251 cells was observed under the invented microscopy. Apoptosis of U251 cells was detected by DNA ladder. Gene expression related with apoptosis was examined by immunohistochemical method. Results Apoptosis rate induced by FC was concentration-dependent (P0.01). When the concentration of FC was over 4. 5 mg/L, the gene expression of bcl-2/bax had no significant differences as compared with control group. Conclusion FC can induce apoptosis of U251 cell, which is not bcl-2/bax-dependent.