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2006•Chinese Journal of Public HealthRequires access

Effect of cadmium on apoptosis and expression of bcl-2,p53,c-myc,c-fos and ras genes in LLC-PK_1 cells

WU Xiao-l

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Abstract

Objective To explore the effect of cadmium on the apoptosis of porcine renal proximal tubule epithelial cells(LLC-PK_1)and the relationship between the apoptosis and the bcl-2,p53,c-myc,c-fos and ras gene expression.Methods The expression of bcl-2,p53,c-myc,c-fos and ras were measured respectively by FCM.Results After LLC-PK_1 cells were incubated with 0,10,20,40?μmol/L CdCl_2 for 4?h or 8?h,the expression of bcl-2,p53,c-myc and ras decreased and there was a significant dose-response relationship(r=-0.910 of bcl-2 for 8?h,r=-0.716,-0.972 of p53 for 4?h,8?h respectively,r=-0.694 of c-myc for 8?h and r=-0.887 of ras for 8?h).Conclusion The apoptosis of LLC-PK_1 cells could be induced by cadmium,and the inhibition of the expression of bcl-2,p53,c-myc and ras would be one possible mechanism.

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Objective To explore the effect of cadmium on the apoptosis of porcine renal proximal tubule epithelial cells(LLC-PK_1)and the relationship between the apoptosis and the bcl-2,p53,c-myc,c-fos and ras gene expression.Methods The expression of bcl-2,p53,c-myc,c-fos and ras were measured respectively by FCM.Results After LLC-PK_1 cells were incubated with 0,10,20,40?μmol/L CdCl_2 for 4?h or 8?h,the expression of bcl-2,p53,c-myc and ras decreased and there was a significant dose-response relationship(r=-0.910 of bcl-2 for 8?h,r=-0.716,-0.972 of p53 for 4?h,8?h respectively,r=-0.694 of c-myc for 8?h and r=-0.887 of ras for 8?h).Conclusion The apoptosis of LLC-PK_1 cells could be induced by cadmium,and the inhibition of the expression of bcl-2,p53,c-myc and ras would be one possible mechanism.

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Available abstract

Objective To explore the effect of cadmium on the apoptosis of porcine renal proximal tubule epithelial cells(LLC-PK_1)and the relationship between the apoptosis and the bcl-2,p53,c-myc,c-fos and ras gene expression.Methods The expression of bcl-2,p53,c-myc,c-fos and ras were measured respectively by FCM.Results After LLC-PK_1 cells were incubated with 0,10,20,40?μmol/L CdCl_2 for 4?h or 8?h,the expression of bcl-2,p53,c-myc and ras decreased and there was a significant dose-response relationship(r=-0.910 of bcl-2 for 8?h,r=-0.716,-0.972 of p53 for 4?h,8?h respectively,r=-0.694 of c-myc for 8?h and r=-0.887 of ras for 8?h).Conclusion The apoptosis of LLC-PK_1 cells could be induced by cadmium,and the inhibition of the expression of bcl-2,p53,c-myc and ras would be one possible mechanism.

Key concepts: Apoptosis, Molecular biology, Gene, Chemistry, Cadmium, Gene expression, P53 protein, Biology

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Effect of cadmium on apoptosis and expression of bcl-2,p53,c-myc,c-fos and ras genes in LLC-PK_1 cells — Research Paper | ScholarLens