Establishment of Regeneration System of Grape Rootstock "1202" in Vitro

The effects of different explant types,different plant growth regulator concentrations and ratios,and different ways of explant placement on the adventitious bud regeneration frequency of grape rootstock 1202 in vitro were studied.The results showed that the regeneration frequency of adventitious bud from the leaf was the highest,being 68.78%.The optimum medium for adventitious bud regeneration from the leaf of grape rootstock 1202 was MS + TDZ 1.0 mg/L + IBA 0.01 mg/L + sucrose 30 g/L+ agar 5.5 g/L(pH 5.8).The best way of leaf placement was placing the abaxial side downward onto the medium.The regenerated adventitious buds were placed in the medium 3/4 MS + 0.35 mg/L IBA to induce rootage,and the rooting rate was 80%.The regenerated plants were cultivated for 35~50 days,then they were transplanted and exercised for acclimatization,and their survival rate reached 90%.