Construction of Leaf Regeneration System of Strawberry Variety "Jingyao"

In this study,the effects of dark time,temperature,AgNO3 concentration,plant growth regulator and plant gel on the regeneration of adventitious bud from leaf explant of strawberry Jingyao were investigated,and an efficient leaf regeneration system was constructed.The results showed that the addition of AgNO3 to the medium had inhibitory effect on the regeneration of adventitious bud from leaf;the following cultivation conditions were in favor of the regeneration of adventitious bud from leaf: 15 days' dark treatment,temperature 28 ℃ and coagulator 2.5 g/L Phytagel;the optimum medium for adventitious bud regeneration was MS supplemented with 1.5 mg/L thidiazuron(TDZ) and 0.3 mg/L indole-3-butyric acid(IBA).Under the above-mentioned optimum conditions,the isolated regeneration rate of adventitious bud from leaf explant reached 76.77%,and the average number of regenerated buds reached 4.3.