Plant Regeneration of Grape Rootstock '5BB' (Vitis berlandieri×V.riparia) in vitro
Tao Jian-min
Abstract
Tao Jian-min
Abstract
The effects of different explant types,basic media,plant growth regulators and their concentration combinations on plant regeneration of grape rootstock'5BB'(Vitis berlandieri×V.riparia) were studied in vitro.The results indicated that the preferable basic medium for inducing adventitious bud from petiole was MS basic medium;The best explant type was petiole which located on the second and the third nodal position.The felicitous growth regulator combination was BA 2.5 mg/L+IBA 0.05 mg/L;The highest regeneration frequency of adventitious bud was 43.33%.
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The effects of different explant types,basic media,plant growth regulators and their concentration combinations on plant regeneration of grape rootstock'5BB'(Vitis berlandieri×V.riparia) were studied in vitro.The results indicated that the preferable basic medium for inducing adventitious bud from petiole was MS basic medium;The best explant type was petiole which located on the second and the third nodal position.The felicitous growth regulator combination was BA 2.5 mg/L+IBA 0.05 mg/L;The highest regeneration frequency of adventitious bud was 43.33%.
Key concepts: Petiole (insect anatomy), Rootstock, Explant culture, Regeneration (biology), Botany, Biology, Plant growth, Horticulture