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Rapid Propagation of Anoectochilus roxburghii

Hanxin Chen

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Abstract

Taking stem segments and apical buds as explants, the optimal culture medium for rapid propagation of Anoectochilus roxburghii were carried out by orthogonal screening experiment. MS + BA 3.5mg/L + KT 1.5mg/L + NAA 0.6mg/L gave the best result for multiplication of adventitious buds and proliferated buds for 3.5 times within 2 months. The growth media supplemented with ABT3 3mg/L increased the number of roots, the length of roots, the height of seedlings and the number of leaves by 12.8%, 34.1%, 13.2% and 22.4%, respectively.

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What this paper is about

Taking stem segments and apical buds as explants, the optimal culture medium for rapid propagation of Anoectochilus roxburghii were carried out by orthogonal screening experiment. MS + BA 3.5mg/L + KT 1.5mg/L + NAA 0.6mg/L gave the best result for multiplication of adventitious buds and proliferated buds for 3.5 times within 2 months. The growth media supplemented with ABT3 3mg/L increased the number of roots, the length of roots, the height of seedlings and the number of leaves by 12.8%, 34.1%, 13.2% and 22.4%, respectively.

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Available abstract

Taking stem segments and apical buds as explants, the optimal culture medium for rapid propagation of Anoectochilus roxburghii were carried out by orthogonal screening experiment. MS + BA 3.5mg/L + KT 1.5mg/L + NAA 0.6mg/L gave the best result for multiplication of adventitious buds and proliferated buds for 3.5 times within 2 months. The growth media supplemented with ABT3 3mg/L increased the number of roots, the length of roots, the height of seedlings and the number of leaves by 12.8%, 34.1%, 13.2% and 22.4%, respectively.

Key concepts: Explant culture, Botany, Horticulture, Multiplication (music), Tissue culture, Biology, Plant growth, Mathematics

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