Study on Optimization of Proliferation and Root Induction Technique of Anoectochilus roxburghii by Orthogonal Design

Taking the test-tube seedling of Anoectochilus roxburghii as material,the optimization of culture medium for adventitious buds proliferation and rooting of Anoectochilus roxburghii were studied by orthogonal design L9(34).The results showed that,the effect for proliferation culture was in the order of 6-BAsucrosemashed bananasNAA,the optimizing medium for proliferation culture was MS+2.5mg/L 6-BA+0.25mg/L NAA+75g/L mashed bananas+28g/L sucrose with the multiplication coefficient of 2.725.The effect of the factor for root induction as follow:the basic culture medium6-BAactivated carbonIBA,the optimizing medium for rooting culture was 1/2MS+0.25mg/L6-BA+5mg/L IBA+2g/L activated carbon and the number of roots per plantlets of 2.405.