Study on anti-angiogenesis in mice with lewis lung carcinoma by Danshen combined with radiotherapy

OBJECTIVE To investigate the effect of herb Danshen combined with radiotherapy on the tumor microvessed density in mice.METHODS Holland Lewis lung carcinoma mice were used as the model and randomly divided into six groups,i.e.control group,low doses of Danshen group,high doses of Danshen group,radiotherapy group,low dose of Danshen with radiotherapy group,high doses of Danshen with radiotherapy group.Each group was administered by intraperitoneal injection from the next day post-inoculation for 12 days.Radiotherapy group,low dose of Danshen with radiotherapy group,high doses of Danshen and radiotherapy group were irradiated with cobalt-60,2Gy·d-1 for 5 days from the seventh post-inoculation,24 h after the final administration,the mice were sacrificed and the tumor weight was recorded to calculate the tumor inhibition rate,the tumor microvessel density(MVD) was measured by immunohistochemistry.RESULTS Microvessel density in Danshen of low dose combined with radiotherapy group compared with radiotherapy and low dose of Danshen group was significantly reduced(P0.05).CONCLUSION Danshen combined with radiotherapy shows obvious inhibition of the angiogenesis in mice with Lewis lung cancer.