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DETERMINATION OF HESPERIDIN IN HAOWAYOU ORAL SOLUTION BY RP HPLC

Ai Lin Chun

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Abstract

OBJECTIVE:To establish an HPLC method for determination of hesperidin in Haowayou oral solution METHODS:The content of hesperidin in Haowayouoral solution was determined by RP HPLC.The condition was ODS column,(5 μm ,150 mm ×6 0 mm); methanol water=38∶62 as mobile phase, and detective wavelength was 285 nm RESULTS:Within the range of 0 18～1 45 μg, hesperidin presented a fine linear relationship between amount and peak area( r =0 9999) The average recovery in sample was 100 1%( n =5, RSD =1 84%) CONCLUSION:The established HPLC method could be used for determination of hesperidin in Haowayou oral solution

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What this paper is about

OBJECTIVE:To establish an HPLC method for determination of hesperidin in Haowayou oral solution METHODS:The content of hesperidin in Haowayouoral solution was determined by RP HPLC.The condition was ODS column,(5 μm ,150 mm ×6 0 mm); methanol water=38∶62 as mobile phase, and detective wavelength was 285 nm RESULTS:Within the range of 0 18～1 45 μg, hesperidin presented a fine linear relationship between amount and peak area( r =0 9999) The average recovery in sample was 100 1%( n =5, RSD =1 84%) CONCLUSION:The established HPLC method could be used for determination of hesperidin in Haowayou oral solution

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Available abstract

OBJECTIVE:To establish an HPLC method for determination of hesperidin in Haowayou oral solution METHODS:The content of hesperidin in Haowayouoral solution was determined by RP HPLC.The condition was ODS column,(5 μm ,150 mm ×6 0 mm); methanol water=38∶62 as mobile phase, and detective wavelength was 285 nm RESULTS:Within the range of 0 18～1 45 μg, hesperidin presented a fine linear relationship between amount and peak area( r =0 9999) The average recovery in sample was 100 1%( n =5, RSD =1 84%) CONCLUSION:The established HPLC method could be used for determination of hesperidin in Haowayou oral solution

Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Chemistry, Linear relationship, Methanol, Medicine, Mathematics

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