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2006•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of hesperidin in Jingouyin by HPLC

Luo Rui-xue

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Abstract

OBJECTIVE To establish an HPLC method for determination of hesperidin in Jinguoyin.METHODS A Agilent zoxbax C_ 18 (15cm×4.6mm,3.5μm) was used with a mobile phase of acetonitrile-0.2%phosphoric acid solution(20∶80),the flow rate was 1.0mL/min,the detection wavelength was at 284nm.RESULTS The calibration curve was linear within the range of 0.35~ 7.0 μg/mL(r=0.999 2),and the average recovery of hesperidin was 99.21%(RSD=1.12%,n=6).CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Jinguoyin.

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OBJECTIVE To establish an HPLC method for determination of hesperidin in Jinguoyin.METHODS A Agilent zoxbax C_ 18 (15cm×4.6mm,3.5μm) was used with a mobile phase of acetonitrile-0.2%phosphoric acid solution(20∶80),the flow rate was 1.0mL/min,the detection wavelength was at 284nm.RESULTS The calibration curve was linear within the range of 0.35~ 7.0 μg/mL(r=0.999 2),and the average recovery of hesperidin was 99.21%(RSD=1.12%,n=6).CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Jinguoyin.

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Available abstract

OBJECTIVE To establish an HPLC method for determination of hesperidin in Jinguoyin.METHODS A Agilent zoxbax C_ 18 (15cm×4.6mm,3.5μm) was used with a mobile phase of acetonitrile-0.2%phosphoric acid solution(20∶80),the flow rate was 1.0mL/min,the detection wavelength was at 284nm.RESULTS The calibration curve was linear within the range of 0.35~ 7.0 μg/mL(r=0.999 2),and the average recovery of hesperidin was 99.21%(RSD=1.12%,n=6).CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Jinguoyin.

Key concepts: Hesperidin, Phosphoric acid, Chromatography, Calibration curve, High-performance liquid chromatography, Chemistry, Linear relationship, Linear range

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