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2006•The Journal of Pharmaceutical PracticeRequires access

Determination of hesperidin in Bainiankang oral liquid by HPLC

Zhao Yong

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Abstract

Objective:To establish the determination of hesperidin in Bainiankang oral liquid.Method:The determination was car- ried out by HPLC with a YWG C_(18)(150mm×4.6mm,5μm)column,acetonitrile-0.25% water(20:80)as mobile phase,detection wavelength was at 285nm,flow rate was 1.0 mL/min.Results:Hesperidin showed a good linear relationship at the range of 5～50mg/L,r=0.999 6.The average recovery was 100.92% and RSD was 1.78%.Conclusion:The method is sensitive,accurate,re- producible,specific and can be used for quality control of Bainiankang oral liquid.

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What this paper is about

Objective:To establish the determination of hesperidin in Bainiankang oral liquid.Method:The determination was car- ried out by HPLC with a YWG C_(18)(150mm×4.6mm,5μm)column,acetonitrile-0.25% water(20:80)as mobile phase,detection wavelength was at 285nm,flow rate was 1.0 mL/min.Results:Hesperidin showed a good linear relationship at the range of 5～50mg/L,r=0.999 6.The average recovery was 100.92% and RSD was 1.78%.Conclusion:The method is sensitive,accurate,re- producible,specific and can be used for quality control of Bainiankang oral liquid.

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Available abstract

Objective:To establish the determination of hesperidin in Bainiankang oral liquid.Method:The determination was car- ried out by HPLC with a YWG C_(18)(150mm×4.6mm,5μm)column,acetonitrile-0.25% water(20:80)as mobile phase,detection wavelength was at 285nm,flow rate was 1.0 mL/min.Results:Hesperidin showed a good linear relationship at the range of 5～50mg/L,r=0.999 6.The average recovery was 100.92% and RSD was 1.78%.Conclusion:The method is sensitive,accurate,re- producible,specific and can be used for quality control of Bainiankang oral liquid.

Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Chemistry, Retention time, Linear range, Linear relationship, Detection limit

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