Association of α-adducin G460Tand AGT M235Tgene polymorphisms with the risk of essential hypertension

Objective To investigate the association between polymorphism of α-adducin G460T,angiotensinogen(AGT) M235T genes and essential hypertension(EH) of Han nationality.Methods MS-PCR and RFLP-PCR were used to detect the polymorphism of α-adducin G460T,AGT M235T genes in 305 EH patients and 305 age-matched,nationality normotensive controls(NT),biochemical index were also detected.Results The G460T polymorphism of α-adducin gene was not significantly associated with EH,the genotype frequencies were 24.60%,52.78% and 22.62% in the patient group and 28.53%,48.85% and 22.62% in the control group respectively.G and T allele frequencies were 50.98%,49.02% in the patient group and 52.95%,47.05% in the control group;The M235T polymorphism of AGT gene was significantly associated with EH,the genotype frequencies were 18.69%,52.46% and 8.85% in the patient group and 9.51%,41.64% and 48.85% in the control group(P0.01),respectively.M and T allele frequencies were 44.92%,55.08% in the patient group and 30.33%,69.67% in the control group(P0.01);There were statistically significant distribution of M235T genotype of AGT and G46OT genotype of α-adducin combined genes with EH except GG genotype of α-adducin;Individuals carrying TT genotype of AGT M235T gene showed high risk of EH(OR=2.35,95%CI=1.69~3.28,P0.01).Conclusion The TT genotype of AGT M235T gene may be related with high risk of EH,and G460T polymorphism of α-adducin gene has no relation to incidence of EH in Han nationality.