Effects of glutathione and alpha-lipoic acid on striatum neuron apoptosis induced by manganese in rats

Objective To study the effects of glutathione(GSH)and alpha-lipoic acid(LA)on rats brain oxidative damage and striatum neuron apoptosis induced by manganese.Methods 32 Wistar rats were divided into control,manganese chloride(MnCl_2),GSH and LA group by weight at random.Control group was injected 0.9% sodium chloride intraperitoneally.Other groups were injected 150?μmol/(kg·bw)MnCl_2 intraperitoneally.2 hours later,control and MnCl_2 group were injected 0.9% sodium chloride subcutaneously,while GSH and LA group were injected 35?μmol/(kg·bw)LA and 1?mmol/(kg·bw)GSH subcutaneously respectively.All groups were administrated 5 times per week,and intervened once every other day.The experimental period was 4 weeks.Then levels of GSH,malonydialdehyde(MDA)and activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in the brain were determined;and striatum neuron apoptosis was detected by TUNEL method.Results Levels of MDA in MnCl_2 group were higher than that in control group,and levels of MDA in GSH and LA group were lower than that in MnCl_2 group.Apoptosis rate of striatum neuron in MnCl_2 group was higher than that in control,GSH and LA group.Conclusion Treatment with GSH and LA on rats could antagonize oxidative damage of brain and striatum neuron apoptosis induced by manganese.