Effects of Sodium Selenite and N-acetylcysteine in Oxidative Damage of Rat Induced by Manganese

Objective To study the effects of sodium selenite(Na2SeO3)and N-acetylcysteine(NAC)on oxidative damage induced by manganese so as to probe into the mechanism of manganese poisoning and to provide criteria for its control.Methods 32 Wistar rats were randomly divided into four groups,i.e.the control,MnCl2,Na2SeO3 intervention and NAC intervention groups.The control group was intraperitoneally(ip)injected with normal saline and other three groups were ip injected with 150 μmol/kg manganese chloride(MnCl2).Two hours later,the control and MnCl2 groups were subcutaneously(sc)injected with normal saline,while Na2SeO3 and NAC intervention groups were sc injected with 10 μmol/kg Na2SeO3 and 1 mmol/kg NAC respectively in every other day.All groups were administrated 5 times a week for four weeks,including a total of 20 times MnCl2 administration,10 times Na2SeO3 and NAC intervention.The levels of reduced glutathione(GSH)and malonydialdehyde(MDA),activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in the liver,brain and kidney of rats were measured.Results Compared with the control group,the levels of MDA of liver,brain and kidney in the manganese exposed group increased significantly,while GSH levels of liver and kidney in the manganese exposed group decreased significantly.Na2SeO3 and NAC intervention caused the decrease of MDA contents in rat brain and kidney treated with manganese.Na2SeO3 intervention resulted in the increase of GSH levels in kidney,reduction of GSH-Px and SOD activities in the liver of rats.NAC intervention caused the reduction of SOD activities in the brain of rats.Conclusions Manganese resulted in oxidative injury in experimental rats.Treatment with Na2SeO3 and NAC can antagonize oxidative damage induced by manganese in certain extent.