Antagonism of Glutathione to Antioxidative Capacity Decrease Induced by Manganese Exposure in Rats

Objective To study the antagonism of glutathione(GSH) to antioxidative capacity decrease induced by manganese exposure in rats.Methods 48 healthy male SD rats were randomly divided into six groups: the blank control,GSH control,MnCl2.4H2O(15 mg/kgand 30 mg/kg),15 mg/kgMnCl2.4H2O+1 mmol/kgGSH and 30 mg/kg MnCl2.4H2O+1 mmol/kg GSH,the treatment was conducted through peritoneal injection.The activity of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px)in the serumand the testis were determined bycolorimetric analysis.Results Compared with the blank control group,the activity of SOD,CAT and GSH-Px in the serum and testis in 30 mg/kg groups significantly decreased(P 0.01).The activity of SOD and CAT in the serum and testis in 30 mg/kg+GSH group decreased significantly(P0.01).Compared with the single manganese treated groups,all antioxidase activities in the serum and testis in 15 mg/kg+ GSH group increased significantly(P0.01).All antioxidase activities in the serum increased significantly(P0.01) and SOD and GSH-Px in the testis increased significantly(P0.01)in 30 mg/kg+GSH group.Pathological examination showed the different degree of denaturation in seminiferous tubule and the decrease of spermatogenic epithelium and spermiogenesis in 15 mg/kg and 30 mg/kg groups.The testicular lesions returned to the normal level in histopathology in 15 mg/kg+GSH group.It was shown that the seminiferous tubule was denatured and the spermiogenesis decreased in 30 mg/kg+GSH group.Conclusion GSH can antagonize antioxidative capacitydecrease induced bymanganese exposure in rats.