Experimental study of the effects of glutathione and alpha-lipoic acid on oxidative response induced by manganese in rats

Thirty-two Wistar rats were randomly divided into control group,MnCl_2 group,GSH group and LA group by body weight.Control group was only injected with 0.9% sodium chloride intraperitoneally,other three groups were all injected with 150μmol/kg manganese chloride(MnCl_2)intraperitoneally first,two hours later,the rats in control group and MnCl_2 group were(injected) with 0.9% sodium chloride subcutaneously,while rats in GSH group and LA group were respectively injected with(1 mmol/kg) GSH and 35 μmol/kg LA subcutaneously,all administration were given 5 times a week for 4 weeks.Then the levels of GSH and malonydialdehyde(MDA),and the activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in liver,brain and kidney of rats were all determined.Results showed that compared with control group,the MDA contents in liver,brain and kidney of the rats from MnCl_2 group were all increased,the GSH contents,GSH-Px and SOD activities were decreased;while the MDA contents in brain and kidney of the rats from GSH group and LA group were declined.Compared with MnCl_2 group,MDA and GSH contents in the liver of rats from GSH group showed some decrease,while SOD and GSH-Px activities increased,and the MDA contents in brain and kidney were decreased;there was the same situation in LA group.It is indicated that GSH and LA both have a certain antagonized effect on oxidative damage caused by manganese in rats.