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2008•Zhonghua zhongyiyao xuekanRequires access

Determination of Hesperidin in Anwei Zhitong Capsules by HPLC

WU Yi-wei

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Abstract

Objective:To set up the assaying method of hesperidin of Tangerine Peel in An Wei Zhi Tong Capsules.Methods:Chromatographic column:Diamonsil C18 5μm,4.6mm×250mm;mobile phase of CH3OH∶HAc∶H2O(35∶4∶61);flow rate:lmL/min;detective wavelength:283nm;column temperature 35℃.Results:Hesperidin has good linear relationship between 0.406～2.842μg,the average recovery rate was 99.39% and RSD was0.7%.Conclusion:The method is accurate,reliability,good reproducibility,can be used in the quality control of the preparation.

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What this paper is about

Objective:To set up the assaying method of hesperidin of Tangerine Peel in An Wei Zhi Tong Capsules.Methods:Chromatographic column:Diamonsil C18 5μm,4.6mm×250mm;mobile phase of CH3OH∶HAc∶H2O(35∶4∶61);flow rate:lmL/min;detective wavelength:283nm;column temperature 35℃.Results:Hesperidin has good linear relationship between 0.406～2.842μg,the average recovery rate was 99.39% and RSD was0.7%.Conclusion:The method is accurate,reliability,good reproducibility,can be used in the quality control of the preparation.

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Available abstract

Objective:To set up the assaying method of hesperidin of Tangerine Peel in An Wei Zhi Tong Capsules.Methods:Chromatographic column:Diamonsil C18 5μm,4.6mm×250mm;mobile phase of CH3OH∶HAc∶H2O(35∶4∶61);flow rate:lmL/min;detective wavelength:283nm;column temperature 35℃.Results:Hesperidin has good linear relationship between 0.406～2.842μg,the average recovery rate was 99.39% and RSD was0.7%.Conclusion:The method is accurate,reliability,good reproducibility,can be used in the quality control of the preparation.

Key concepts: Hesperidin, Chromatography, Chemistry, Reproducibility, High-performance liquid chromatography, Recovery rate, Linear relationship, Mathematics

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