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2008•Strait Pharmaceutical JournalRequires access

Determination of hesperidin in Xiangsha Weiling pills by HPLC

Chen Fu

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Abstract

OBJECTIVE To determine hesperidin in Xiangsha Weiling pills by HPLC.METHODS HPLC was used in the quantitative analysis of hesperidin in the sample.The separation was performed on Diamonsil C18(4.6×250 mm,5μm) column with a mixture of methanol-2% acetic acid glacial(38∶62)as mobile phase.The detection wavelength was set at 284 nm and the flow rate was 1.0mL·min-1.RESULTS The linear range of hesperidin was 0.1028～1.028μg(r=0.9999),the average recovery was 96.92%,RSD=0.98%(n=6).CONCLUSION The method is simple,reliable,and good to determine hesperidin.

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What this paper is about

OBJECTIVE To determine hesperidin in Xiangsha Weiling pills by HPLC.METHODS HPLC was used in the quantitative analysis of hesperidin in the sample.The separation was performed on Diamonsil C18(4.6×250 mm,5μm) column with a mixture of methanol-2% acetic acid glacial(38∶62)as mobile phase.The detection wavelength was set at 284 nm and the flow rate was 1.0mL·min-1.RESULTS The linear range of hesperidin was 0.1028～1.028μg(r=0.9999),the average recovery was 96.92%,RSD=0.98%(n=6).CONCLUSION The method is simple,reliable,and good to determine hesperidin.

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Available abstract

OBJECTIVE To determine hesperidin in Xiangsha Weiling pills by HPLC.METHODS HPLC was used in the quantitative analysis of hesperidin in the sample.The separation was performed on Diamonsil C18(4.6×250 mm,5μm) column with a mixture of methanol-2% acetic acid glacial(38∶62)as mobile phase.The detection wavelength was set at 284 nm and the flow rate was 1.0mL·min-1.RESULTS The linear range of hesperidin was 0.1028～1.028μg(r=0.9999),the average recovery was 96.92%,RSD=0.98%(n=6).CONCLUSION The method is simple,reliable,and good to determine hesperidin.

Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Linear range, Detection limit, Medicine

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