Effects of Bushen Zhuangjin decoction(补肾壮筋汤) on apoptosis of chondrocyte and expression of PCNA in earlier period experimental osteoarthritis of rabbit

Objective:To observe the effects of Bushen Zhuangjin decoction(补肾壮筋汤) on apoptosis of chondrocyte,expression of proliferation cell nuclear antigen(PCNA)in earlier period of experimental osteoarthritis of rabbit,and analyze the relation between of them.Methods:Forty Livor rabbits were divided into four groups randomly:Normal group,model contrast group,Chinese herb group,sham operation group.There were ten rabbits in every group.OA model was induced in rabbits by Hulth's method.After three weeks Chinese herb group and sham operation group(which only cut out the skin)fed with Bushen Zhuangjin decoction,normol group and model contrast group fed with 0.9%NS at the same time.Specimens were harvested at the 8th week.Cartilage sections were analyzed by immunohistochemistry for the PCNA,TUNEL for apoptosis.Observe the change of morphology of chondrocyte by light microscopic and transmission electron microscopic.Results:Apoptosis cell index and cell proliferation index have significant difference between Chinese herb group and model contrast group(P0.05).Conclusion:Bushen Zhuangjin decoction can decrease the apoptosis of chondrocyte,increase of proliferation chondrocyte.There was therapeutical effect in the earlier period of experimental osteoathritis.