Empirical Study on Bushen Zhuangjin Decoction Inhibiting Inflammatory Cytokine Expression Experiments to Delay the Degeneration of Articular Cartilage

Objective:To investigate the inhibitive effects of Bushen Zhuangjin Decoction on inflammatory cytokine expression to delay the degeneration of articular cartilage.Methods:40 SD rats of 8 weeks old were randomly divided into the normal group(10 rats) and the model group(30 rats).After replication of osteoarthritis models with 4% mass fraction of papain,the model group were randomly and equally divided into the model control group,the Bushen zhuangjin Decottion group,and the positive control group.The normal group were routinely feed;the model control group were given lavage with normal saline;the BuShen Zhuangjin Decoction group were given Bushen Zhuangjin Decoction(10.5 g·kg-1·d-1),and the positive control group were given lavage with Glucosamine Hydrochloride Capsules solution(150 mg·kg-1·d-1).All above treatments were done for 12 weeks.HE and safranin O-fast green staining were used to observe the changes of cartilagemorphology.ELISA was used to detect the content of MMP-3,IL-1β,TNF-α and HA in synovial fluid.Results:The Bushen Zhuangjin Decoction group was better than the positive control group on the aspects of the contents of proteoglycans in the cartilage matrix,the denaturation of collagen fiber,and the degeneration of cartilage.The contents of IL-1β,TNF-α,and MMP-3 in synovia of the Bushen Zhuangjin Decoction group and the positive control group were significantly lower than those in the model control group(P 0.05).Conclusion:Bushen Zhuangjin Decoction can delay osteoarthritis cartilage degeneration by inhibiting the expression of inflammatory cytokines.