Influence of Bushen Zhuangjin Decoction on cartilage morphology and skeleton proteins of ROCK,Cofilin,Phospho-Cofilin,LIMK1 and Phospho-LIMK1 in rabbits with knee osteoarthritis

Objective:To explore the influence of Bushen Zhuangjin Decoctioin on cartilage morphology and skeleton proteins in rabbits with knee osteoarthritis.Methods:Forty-eight 3 month old New Zealand rabbits were randomly divided into 4groups as control group,model group,low and high dosage of Bushen Zhuangjin Decoction groups,and with 12 rabbits in each group.The rabbit model of osteoarthritis in the early stage was established by using Hulth method.Rabbits in control group were fed conventionally,rabbits in model group received gavage administration with normal saline,rabbits inlow and high dosage of Bushen Zhuangjin Decoction groups received gavage administration with 10.5g/kg,once a day and Bushen Zhuanggu Decoction10.5g/kg,twice a day,and all the treatments were lasted for 4 weeks.The changes in shape and structure of cartilage were observed by using HE staining method,and the changes in the concentration of proteins as ROCK,Cofilin,Phospho-Cofilin,LIMKI and Phospho-LIMKI in cartilage were tested by using Western blot method.Results:The degeneration grade of articular cartilage of rabbits in low and high dosage of Bushen Zhuangjin Decoction groups was slighter than that of rabbits in model group(P0.05).The expression of proteins as ROCK,Cofilin,Phospho-Cofilin,LIMKI and Phospho-LIMKI in rabbits of low and high dosage of Bushen Zhuangjin Decoction groups was lower than that of rabbits in model group,but which was higher than that of rabbits in control group(P0.05).Conclusion:Bushen Zhuangjin Decoction could inhibit the development of cytoskeleton damage in cartilage and slower the articular degeneration by regulating the expression of related proteins of the cytoskeleton signal pathway.